The effects of vascular smooth muscle caldesmon on force production by ‘desensitised’ skeletal muscle fibres

FEBS Letters

Marston, Steven B.¹  Taggart, Michael J.¹ 

[1] National Heart and Lung Institute, Dovehouse Street, London SW3 6LY, England

关键词: Caldesmon;    Ca2+ regulation;    Contraction;    (Skeletal muscle;    Smooth muscle);   

DOI  :  10.1016/0014-5793(88)81009-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Smooth muscle caldesmon inhibits actomyosin MgATPase in the absence of Ca²⁺ and is the key regulatory component of Ca²⁺-regulated thin filaments. We now show that caldesmon can affect contractility as well. Glycerinated skeletal muscle fibres were treated so as to produce substantial contractions without Ca²⁺ as an activator. Addition of caldesmon caused reductions in force in a concentration- and time-dependent manner. Perfusion with caldesmon concentrations for < 5 min gave up to 48% reduction in isometric tension with a half-maximal effect at 1.5 μm caldesmon. Perfusion with 15 μM caldesmon for 30 min gave an irreversible tension drop.

【 授权许可】

Unknown