FEBS Letters | |
The effects of vascular smooth muscle caldesmon on force production by ‘desensitised’ skeletal muscle fibres | |
Marston, Steven B.1  Taggart, Michael J.1  | |
[1] National Heart and Lung Institute, Dovehouse Street, London SW3 6LY, England | |
关键词: Caldesmon; Ca2+ regulation; Contraction; (Skeletal muscle; Smooth muscle); | |
DOI : 10.1016/0014-5793(88)81009-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Smooth muscle caldesmon inhibits actomyosin MgATPase in the absence of Ca2+ and is the key regulatory component of Ca2+-regulated thin filaments. We now show that caldesmon can affect contractility as well. Glycerinated skeletal muscle fibres were treated so as to produce substantial contractions without Ca2+ as an activator. Addition of caldesmon caused reductions in force in a concentration- and time-dependent manner. Perfusion with caldesmon concentrations for < 5 min gave up to 48% reduction in isometric tension with a half-maximal effect at 1.5 μm caldesmon. Perfusion with 15 μM caldesmon for 30 min gave an irreversible tension drop.
【 授权许可】
Unknown
【 预 览 】
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RO201912020291432ZK.pdf | 227KB | download |