Tetrameric structure of the nonactivated glucocorticoid receptor in cell extracts and intact cells

FEBS Letters

Segnitz, Bernd¹  Gehring, Ulrich¹  Busch, Willi¹  Rexin, Martin¹ 

[1] Institut für Biologische Chemie der Universität, Im Neuenheimer Feld 501, 6900 Heidelberg, FRG

关键词: Chemical crosslinking;    Dexamethasone mesylate;    Dimethyl suberimidate;    Glucocorticoid receptor;    Subunit structure;    DMS;    dimethyl suberimidate;    nti;    receptor of ‘increased nuclear transfer’;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(88)81068-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mouse lymphoma cells contain a nonactivated glucocorticoid receptor of M _r∼330000 which is heteromeric in nature and is unable to bind to DNA. Following affinity labeling of the steroid-binding subunit and subsequent cross-linking with dimethyl suberimidate at various times either in cell extracts or in intact cells, a series of labeled bands was detected in SDS gels. From the molecular masses of completely and partially cross-linked complexes we conclude that the large nonactivated receptor is a tetramer composed of two 90 kDa subunits, one 50 kDa polypeptide and one steroid-binding subunit.

【 授权许可】

Unknown