Partial sequence of the rat heavy neurofilament polypeptide (NF‐H) Identification of putative phosphorylation sites

FEBS Letters

Breen, Kieran C.¹  Robinson, Philip A.¹  Anderton, Brian H.¹  Wion, Didier¹ 

[1] Dept of Immunology, St. Georges Hospital Medical School, Cranmer Terrace, London SW17 ORE, England

关键词: Neurofilament;    cDNA sequence;    Phosphorylation site;    Interspecies comparison;    (Rat brain);   

DOI  :  10.1016/0014-5793(88)81064-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 3 kb cDNA clone has previously been isolated in this laboratory corresponding to the rat heavy neurofilament polypeptide (NF-H). This clone, equivalent to approximately 70% of the total mRNA of the protein has been sequenced and shown to contain the carboxy-terminal region of the message. This contains 51 of the Lys-Ser-Pro repeat triplets which are reported to be the site of neurofilament phosphorylation. The sequence obtained was subsequently compared to those of mouse and human NF-H, showing a homology of approximately 85%. There is, however, one region which is variable between the species, this being the highly phosphorylated region of the protein containing the Lys-Ser-Pro triplet repeat.

【 授权许可】

Unknown