Metallothionein RNA levels in HL‐60 cells Effect of cadmium, differentiation, and protein kinase C activation

FEBS Letters

Tyers, Michael²  Harley, Calvin B.²  Hanke, Tomas¹ 

[1] Department of Pathology, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada;Department of Biochemistry, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada

关键词: Differentiation;    Metallothionein;    RNA;    Cadmium;    Protein kinase C;    (HL-60 cell);    MT;    metallothionein;    TPA;    12-O-tetradecanoyl-phorbol 13-acetate;    Ra;    retinoic acid;    PKC;    protein kinase C (EC 2.7.1.-);   

DOI  :  10.1016/0014-5793(88)81051-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Metallothionein (MT) gene transcription is regulated in a developmental and tissue-specific manner by metal ions and other agents. We examined MT expression in the human promyelocytic leukemia cell line HL-60 during differentiation along macrophage and neutrophil lineages. All HL-60 phenotypes showed similar basal levels of MT RNA with significant induction following exposure to Cd²⁺ but not activators of PKC. MT RNA did not correlate with growth state or with known levels of PKC activity, thus our data do not support a role for MT in HL-60 differentiation or for PKC in MT expression.

【 授权许可】

Unknown