Location of a region of the muscarinic acetylcholine receptor involved in selective effector coupling

FEBS Letters

Akiba, Isamu¹  Mishina, Masayoshi¹  Nakai, Junichi¹  Bujo, Hideaki¹  Numa, Shosaku¹  Kubo, Tai¹ 

[1] Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan

关键词: Muscarinic acetylcholine receptor subtype;    Chimaeric receptor;    cDNA expression;    Effector coupling;    Selective antagonist;    (Xenopus oocyte);    mAChR;    muscarinic acetylcholine receptor;    ACh;    acetylcholine;    NMS;    N-methylscopolamine;    QNB;    quinuclidinyl benzilate;   

DOI  :  10.1016/0014-5793(88)81043-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chimaeric muscarinic acetylcholine receptors (mAChR) in which corresponding portions of mAChR I and mAChR II are replaced with each other have been produced in Xenopus oocytes by expression of cDNA constructs encoding them. Functional analysis of the chimaeric mAChRs indicates that a region mostly comprising the putative cytoplasmic portion between the proposed transmembrane segments V and VI is involved in selective coupling of mAChR I and mAChR II with different effector systems. In contrast, the exchange of this region between mAChR I and mAChR II does not significantly affect the antagonist binding properties of the two mAChR subtypes.

【 授权许可】

Unknown