Inhibition by amiloride and by Na+‐depletion of A23187‐stimulated arachidonic acid and histamine release from rat mast cells

FEBS Letters

Linnebjerg, Helle¹  Jensen, Benny¹  Hansen, Harald S.¹ 

[1] PharmaBiotec Research Center, Biochemical Laboratory, Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen Ø, Denmark

关键词: Amiloride;    Arachidonic acid;    Na+/H+ exchange;    Phospholipase A2;    Histamine;    (Mast cell);    H-7;    1-(5-isoquinolinylsulphonyl)-2-methylpiperazine;    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid;    [14C]AA;    [14C]arachidonic acid;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    PS;    phosphatidylserine;    PI;    phosphatidylinositol;    PA;    phosphatidic acid;    TG;    triacylglycerol;    DG;    diacylglycerol;    MG;    monoacylglycerol;    PGD2;    prostaglandin D2;    AA;    arachidonic acid;   

DOI  :  10.1016/0014-5793(88)80361-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat peritoneal mast cells, labelled with [¹⁴C]arachidonic acid, released histamine and [¹⁴C]arachidonic acid upon the addition of A23187. The release induced by low concentrations of A23187 was suppressed by removal of extracellular Na⁺ and by addition of the Na⁺/H⁺ exchange inhibitor, amiloride. Addition of protein kinase C inhibitors resulted in only a modest decrease of [¹⁴C]arachidonic acid and histamine release. [¹⁴C]Arachidonic acid was hydrolyzed from both phosphatidylcholine and triacylglycerol. Amiloride inhibited only the hydrolysis from phosphatidylcholine. It is suggested that an Na⁺/H⁺ exchange is stimulated secondary to an A23187-induced increase in intracellular calcium concentration. Increased Na⁺/H⁺ exchange seems to facilitate the activation by intracellular calcium ions of phospholipase A₂ as well as of histamine secretion.

【 授权许可】

Unknown