DNA‐binding protein(s) interacts with a conserved nonameric sequence in the upstream regions of wheat histone genes

FEBS Letters

Mikami, Koji¹  Nakayama, Takuya¹  Takase, Hisabumi¹  Kawata, Takefumi¹  Tabata, Tetsuya¹  Iwabuchi, Masaki¹ 

[1] Department of Biology, Faculty of Science, Hokkaido University, Sapporo 060, Japan

关键词: Histone gene;    Nonameric motif;    DNA-binding protein;    Mobility shift assay;    DNase I footprint;    (Wheat);   

DOI  :  10.1016/0014-5793(88)80943-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A nuclear protein(s), HBP-2, that binds to the upstream region of the wheat histone H4 gene was identified from a fractionated nuclear extract of wheat germ by DNase I footprinting. The DNase I-protected region contained the conserved nonameric motif, CATCCAACG. Cross-competition experiments that used the mobility shift assay showed that this nuclear protein(s) binds specifically to the upstream sequence that has been postulated to be a cis element of the wheat H3 gene. Our findings suggest that this DNA-binding protein(s) may be a trans-acting factor in the regulation of the transcription of wheat histone genes.

【 授权许可】

Unknown