A cAMP‐triggered release of a hormone‐like peptide

FEBS Letters

Tauber-Finkelstein, Miriam¹  Riven-Kreitman, Rivka¹  Ribon, Vered¹  Shaltiel, Shmuel¹ 

[1] Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: cyclic AMP;    cyclic AMP-dependent protein kinase;    Cell-cell signaling;    Immobilized cyclic AMP;    Peptide hormone;    (B-lymphoma cell line);    HLF;    hormone-like factor;    PKA;    CAMP-dependent protein kinase;    SDS-PAGE;    polyacrylamide gel electrophoresis in the presence of SDS;   

DOI  :  10.1016/0014-5793(88)80926-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Preparations of the catalytic subunit of cAMP-dependent protein kinase from rabbit skeletal muscle, which appear to be homogeneous by SDS-polyacrylamide gel electrophoresis, were often found to contain a hormone-like factor (HLF) which causes an immediate rise, then a decline of intracellular cAMP in a B-lymphoma cell line. Active HLF is released when the fractions that contain it in an inactive form are incubated with cAMP prior to chromatography, or passed through an immobilized cAMP column. HLF seems to be a peptide: it loses its cell-stimulating capability after proteolysis and has an apparent molecular mass of 2.2 – 2.5 kDa.

【 授权许可】

Unknown