Estrogen‐controlled gene expression in tissue culture cells by zearalenone

FEBS Letters

Mayr, Ulrich E.¹ 

[1] Institut für Tierernährung, Universität Hohenheim, D-7000 Stuttgart 70, FRG

关键词: Mycotoxin;    Zearalenone;    Estrogen receptor activation;    Gene induction;    Zearalenone bioassay;    (MCF-7;    Le42 cell);    CAT;    chloramphenicol acetyltransferase;    ER;    estrogen receptor;    ERE;    estrogen responsive element;   

DOI  :  10.1016/0014-5793(88)80921-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In two estrogen-sensitive cell lines, Le42 and MCF-7, the estrogenic potential of the nonsteroidal mycotoxin zearalenone has been investigated. The chloramphenicol acetyltransferase (CAT) gene expression in Le42 cells is induced by zearalenone after transfection with a CAT-gene construct controlled by an estrogen responsive element [(1986) Cell 46, 1053–1061]. In MCF-7 cells zearalenone induces at least 2 exoproteins (52 and 160 kDa) which are estrogen-specific [(1980) Cell 20, 353–362). These data suggest that zearalenone acts by activating the estrogen receptor. Due to the high sensitivity of these cell lines for zearalenone both test systems are proposed as assays for a quantitative estimation of the biological (estrogenic) activity of this widespread mycotoxin.

【 授权许可】

Unknown