Tumor necrosis factor stimulates prostaglandin production and cyclic AMP levels in rat cultured mesangial cells

FEBS Letters

Perez, Joelle¹  Ardaillou, Raymond¹  Baud, Laurent¹  Friedlander, Gérard² 

[1] INSERM 64, Hôpital Tenon, Paris, France;INSERM 251, Faculté de Médecine Xavier Bichat, Paris, France

关键词: Tumor necrosis factor;    Prostaglandin;    Adenosine cyclic monophosphate;    Glomerular mesangium;   

DOI  :  10.1016/0014-5793(88)80543-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human recombinant tumor necrosis factor-α (TNF) was found to stimulate the production of prostaglandins (PG) by cultured rat mesangial cells. This effect was demonstrable from 6 h, was dose dependent and affected the synthesis of PGE₂, PGF_2α, and 6-keto-PGF_1α. It required both RNA and protein synthesis but was not associated with a modification of cell proliferation. TNF also stimulated adenosine 3′–5′ cyclic monophosphate (cAMP) levels in the mesangial cell culture medium. Indomethacin suppressed the effect of TNF on PGs but only reduced that on cAMP, indicating that PG production partly mediates the increase in cAMP. These findings demonstrate that mesangial cells can be a target for TNF and that the mechanism of TNF action includes stimulation of both PG production and cAMP levels.

【 授权许可】

Unknown