Electrogenic photocycle of the 13‐cis retinal‐containing bacteriorhodopsin with an M intermediate involved

FEBS Letters

Zorina, V.V.¹  Drachev, L.A.¹  Kaulen, A.D.¹  Skulachev, V.P.¹ 

[1] A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Bacteriorhodopsin;    Photocylce;    13-cis-Retinal;    Light-dark adaptation;    M intermediate;    (Halobacterium halobium);   

DOI  :  10.1016/0014-5793(88)80533-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been found that bacteriorhodopsin containing 13-cis-retinal as the chromophore (13-cis-bR) is competent in a one-photon photocycle which involves an M-type intermediate. Such an effect can be shown in 13-cis-Br obtained by means of 13-cis-retinal treatment of white bacterioopsin membrane sheets, as well as in dark-adapted purple sheets, solubilized Br and Br proteoliposomes. The 13-cis-Br photocycle with the M intermediate is found to be proton-motive, the electrogenic phases resembling those of the all-trans-Br photocycle. All of these effects are observed only when the Ph is above some critical value, namely Ph 8.5–9.5 for the Br sheets, 7.6 for proteoliposomes and 7.0 for the solubilized Br.

【 授权许可】

Unknown