Expression of four types of human tyrosine hydroxylase in COS cells

FEBS Letters

Kaneda, Norio³  Ishii, Akira³  Nagatsu, Toshiharu³  Kurosawa, Yoshikazu²  Kiuchi, Kazutoshi¹  Kobayashi, Kazuto³  Fujita, Keisuke² 

[1] Radioisotope Center Medical Branch, Nagoya University School of Medicine, Nagoya 466, Japan;Institute for Comprehensive Medical Science, Fujita-Gakuen Health University School of Medicine, Toyoake 470-11, Japan;Department of Biochemistry, Radioisotope Center Medical Branch, Nagoya University School of Medicine, Nagoya 466, Japan

关键词: Tyrosine hydroxylase;    Catecholamine synthesis;    Transient expression;    Alternative splicing;    (Human;    COS cell);    TH;    tyrosine hydroxylase;    ECO-MOL;    ecotropic Moloney virus;    LTR;    long terminal repeat;    PAGE;    polyacrylamide gel electrophoresis;    6-MPH4;    6-methyl-5;    6;    7;    8-tetrahydropteridine;   

DOI  :  10.1016/0014-5793(88)80526-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Alternative splicing from a single gene produces four kinds of human tyrosine hydroxylase (types 1–4) which have structural diversity only in the N-terminal region. We attempted expression of the type 1–4 enzymes in COS cells and performed kinetic analyses. All had enzymatic activities. The K _m values of the four types for L-tyrosine and 6-methyl-5,6,7,8-tetrahydropteridine were similar, although their relative homospecific activities were clearly different. The type 1 enzyme displayed the highest activity.

【 授权许可】

Unknown