Total synthesis of the cystatin α gene and its expression in E. coli

FEBS Letters

Yamato, Masayuki¹  Katunuma, Nobuhiko¹  Ike, Yoshimasa²  Kominami, Eiki¹ 

[1] Division of Enzyme Chemistry Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan;Central Research Institute Mitsui Toatsu Chemical, Inc., Biotechnology Laboratory, Mobara, Chiba 297, Japan

关键词: Cystatin α;    Cysteine proteinase inhibitor;    Synthetic gene;    (E. coli);   

DOI  :  10.1016/0014-5793(88)80238-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A gene encoding cystatin α has been chemically synthesized, cloned and expressed in E. coli. The gene of 318 base pairs was assembled by enzymatic ligation of 19 oligonucleotides and cloned into a pBR322-derived expression plasmid down stream of the tac promoter. The expression product of the synthetic gene has been purified by Sephadex G-50 column chromatography and shown to have the same properties as those of the authentic protein isolated from rat epidermis.

【 授权许可】

Unknown