Inhibition of protein breakdown by glutamine in perfused rat skeletal muscle

FEBS Letters

MacLennan, Peter A.¹  Watt, Peter W.¹  Smith, Kenneth¹  Weryk, Brian¹  Rennie, Michael J.¹ 

[1] Department of Physiology, University of Dundee, Dundee DD1 4HN, Scotland

关键词: Glutamine;    Anabolism;    Myofibrillar protein;    Soluble protein;    (Muscle);   

DOI  :  10.1016/0014-5793(88)80186-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have assessed the effects of glutamine (Gln) availability on protein breakdown in perfused rat hindlimb by measuring net phenylalanine (Phe) production (an index of protein balance), the dilution of [¹⁵N]Phe labelling (an index of mixed protein breakdown) and rate of production of 3-methylhistidine (3-MeH) (an index of myofibrillar breakdown). 15 mM Gln significantly inhibited net protein loss and protein breakdown compared to rates obtained in its absence (net protein loss, 200±230 vs 2080±200 nmol Phe/hindlimb per h; protein breakdown, 4566±480 vs 1614±180 nmol Phe/hindlimb per h; both p<0.01). Insulin (100 μU/ml) inhibited protein breakdown but less than Gln. The effects on protein breakdown of Gln and insulin together were not additive, suggesting a common mode of action. Production of 3-MeH (mean 20.3±2.8 nmol/hindlimb per h) was unaffected by Gln or insulin. Gln appears to inhibit protein breakdown of soluble rather than myofibrillar protein in muscle.

【 授权许可】

Unknown