Induction of eIF‐4E phosphorylation by the addition of L‐pyrroline‐5‐carboxylic acid to rabbit reticulocyte lysate

FEBS Letters

Ray, Bimal K.³  Thach, Robert E.³  Abramson, Richard D.²  Mick, Steven J.¹  Hagedorn, Curt H.¹  Merrick, William C.² 

[1] Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA;Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA;Department of Biology, Washington University, St. Louis, MO 63130, USA

关键词: eIF-4E;    eIF-4F;    mRNA cap;    L-pyrroline-5-carboxylic acid;    Translation;    Glutathione;    eIF-4F;    eukaryotic initiation factor-4F (p25;    p48 and p220 subunits);    eIF-4E;    eukaryotic initiation factor-4E (isolated p25);    eIF-2;    eukaryotic initiation factor-2;    eIF-4A;    eukaryotic initiation factor-4A;    eIF-4B;    eukaryotic initiation factor-4B;    PAGE;    polyacrylamide gel electrophoresis;    IEF;    isoelectric focusing;    DTE;    dithioerythritol;    P5C;    L-pyrroline-5-carboxylic acid;    PP-ribose-P;    5-phosphoribosyl 1-pyrophosphate;   

DOI  :  10.1016/0014-5793(88)80082-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of L-pyrroline-5-carboxylic acid to reticulocyte lysates inhibits protein synthesis and induced phosphoproteins of 25 and 14 kDa. The 25 kDa phosphoprotein had the same M _r and pI as phosphorylated eIF-4E. Incubation of lysates with L-pyrroline-5-carboxylic acid did not alter the crosslinking of eIF-4E to reovirus mRNA caps. These results suggest that modifications of the translational apparatus other than eIF-4E phosphorylation may mediate the inhibitory effect seen with L-pyrroline-5-carboxylic acid and/or that phosphorylation of eIF-4E may effect functions subsequent to its interaction with the mRNA cap such as protein-protein interactions with other cap-specific translation factors.

【 授权许可】

Unknown