Differentiation‐associated changes of cation‐transport activities in myeloid leukemic cell lines

FEBS Letters

Englender, Talma¹  Aviram, Adina¹  Rephaeli, Ada¹  Shaklai, Mati¹ 

[1] Hematology Division, Beilinson Medical Center, Petach-Tikva 49100 and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

关键词: Ca2+;    86Rb+ transport;    Retinoic acid;    (HL-60 leukemic cell;    U-937 leukemic cell);    MEL;    murine erythroleukemia cell line;    NBT;    nitro-blue tetrazolium;    PMA;    phorbol 12-myristate-13-acetate;    [Ca2+ i;    cytosolic free calcium concentration;    fura-2AM;    fura-2 acetoxymethyl ester;   

DOI  :  10.1016/0014-5793(88)80065-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5–10-fold increase in ⁴⁵Ca²⁺ uptake. The increased ⁴⁵Ca²⁺ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca²⁺ uptake in differentiating cells was also demonstrated using the fluorescent probe, fura-2 acetoxymethyl ester. The increased ⁴⁵Ca²⁺ uptake was accompanied by a decrease in ouabain-insensitive and -sensitive ⁸⁶Rb⁺ uptake. Furthermore, correlation between the changes in these activities was observed. Modulation of extracellular pH affected differentiation: higher pH increased the extent of differentiation.

【 授权许可】

Unknown