FEBS Letters | |
Differentiation‐associated changes of cation‐transport activities in myeloid leukemic cell lines | |
Englender, Talma1  Aviram, Adina1  Rephaeli, Ada1  Shaklai, Mati1  | |
[1] Hematology Division, Beilinson Medical Center, Petach-Tikva 49100 and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel | |
关键词: Ca2+; 86Rb+ transport; Retinoic acid; (HL-60 leukemic cell; U-937 leukemic cell); MEL; murine erythroleukemia cell line; NBT; nitro-blue tetrazolium; PMA; phorbol 12-myristate-13-acetate; [Ca2+ i; cytosolic free calcium concentration; fura-2AM; fura-2 acetoxymethyl ester; | |
DOI : 10.1016/0014-5793(88)80065-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Induction of differentiation in HL-60 and U-937 leukemic cell lines, resulted in 1.5–10-fold increase in 45Ca2+ uptake. The increased 45Ca2+ uptake in the differentiating cells was inhibited by verapamil, cromolyn and amiloride. Elevation in Ca2+ uptake in differentiating cells was also demonstrated using the fluorescent probe, fura-2 acetoxymethyl ester. The increased 45Ca2+ uptake was accompanied by a decrease in ouabain-insensitive and -sensitive 86Rb+ uptake. Furthermore, correlation between the changes in these activities was observed. Modulation of extracellular pH affected differentiation: higher pH increased the extent of differentiation.
【 授权许可】
Unknown
【 预 览 】
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