Production of a specific antibody against pyruvate kinase type M2 using a synthetic peptide

FEBS Letters

Staal, Gerard E.J.¹  Oude Weernink, Paschal A.¹  Rijksen, Gert¹ 

[1] Department of Haematology, Laboratory of Medical Enzymology, University Hospital Utrecht, Catharijnesingel 101, 3511 GV Utrecht, Netherlands

关键词: Pyruvate kinase;    Isozyme;    Immunoblotting;    Peptide antibody;    PK;    pyruvate kinase;    AMTC;    anaplastic medullary thyroid carcinoma (rat);    DMTC;    differentiated medullary thyroid carcinoma (rat);    KLH;    keyhole limpet hemocyanin;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    EDAC;    1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride;   

DOI  :  10.1016/0014-5793(88)80063-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The pyruvate kinase isozymes M1 and M2 are structurally and immunologically closely related. To obtain an antibody which discriminates between these two forms, a synthetic tetradecapeptide with a sequence specific for pyruvate kinase type M2 from rats was constructed. Antisera from rabbits, immunized with this peptide, reacted specifically with the M2-type holoenzyme of both rat and human origin, and did not cross-react with the M1-type isozyme. This was established by immunoblot analysis, both under dissociating and non-dissociating conditions.

【 授权许可】

Unknown