Involvement of proteasomes (multicatalytic proteinase) in ATP‐dependent proteolysis in rat reticulocyte extracts

FEBS Letters

Tanaka, Keiji¹  Ichihara, Akira¹ 

[1] Institute for Enzyme Research, University of Tokushima, Tokushima 770, Japan

关键词: Multicatalytic proteinase;    ATP;    Proteolysis;    Reticulocyte;    Proteasome;   

DOI  :  10.1016/0014-5793(88)80306-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of proteasomes, particles with latent multicatalytic proteinase, in ATP-dependent proteolysis in rat reticulocyte extracts was examined. Removal of proteasomes from the extracts by immunoprecipitation caused almost complete inhibition of ATP-dependent degradation of [³H]methylcasein, without affecting ATP-independent proteolysis. Peptide fragments of [³H]casein, obtained by cyanogen bromide cleavage, were rapidly degraded in an ATP-independent fashion and this activity was not affected by removal of the proteasomes. These results suggest that proteasomes are involved in ATP-dependent proteolysis in the extracts and that they catalyze the initial cleavage of large proteins.

【 授权许可】

Unknown