Membrane excitability expressed in human neuroblastoma cell hybrids

FEBS Letters

Gilbert, Fred¹  Silver, Ian A.³  Giovanni, Maria Y.¹  Glick, Mary Catherine² 

[1] Department of Pediatrics, Mt. Sinai School of Medicine, NY 10029 USA;Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA;Department of Pathology, Medical School, University of Bristol, Bristol BS8 1TD, England

关键词: Voltage-sensitive Na+ channel;    Karyotype;    Neurotoxin;    Cell hybrid;    (Human;    Mouse);    Hybrid cell lines: N-5;    N4BTP;    N-13;    N4HTP-13;    N-8;    N4DTP-8;    and N-10;    N2DTP-10;    TPMP;    tritiated triphenylmethyl phosphonium bromide;    HAT;    hypoxanthine;    aminopterin and thymidine;    VTD;    veratridine;    TTX;    tetrodotoxin;    ScTX;    Leiurus quinquestriatus scorpion venom;   

DOI  :  10.1016/0014-5793(88)80281-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The voltage-sensitive Na⁺ channel is responsible for the action potential of membrane electrical excitability in neuronal tissue. Three methods were used to demonstrate the presence of neurotoxin-responsive Na⁺ channels in two hybrid cell lines resulting from the fusion of excitable human neuroblastoma cells with mouse fibroblasts. Only one of the two electrically active hybrid cell lines maintained the sensitivity of the neuroblastoma parent to tetrodotoxin (TTX). The other hybrid, although electrically active, was not responsive to TTX or scorpion venom. Comparisons of the patterns of expression of membrane excitability and of chromosome complements in these human neuroblastoma cell hybrids suggest that the phenotype of membrane excitability is composed of genetically distinct elements.

【 授权许可】

Unknown