Uranyl salts as photochemical agents for cleavage of DNA and probing of protein‐DNA contacts

FEBS Letters

Jeppesen, Claus²  Buchardt, Ole¹  Nielsen, Peter E.² 

[1] The Bioganic Group, Chemical Laboratory II, The H.C. Ørsted Institute, Universitetsparken 5, DK-2100 Copenhagen Ø, Denmark;Department of Biochemistry B, The Panum Institute, University of Copenhagen, Blegdamsvej 3c, DK-2200 Copenhagen N Denmark

关键词: Photofootprinting;    Uranyl ion;    DNA;    Photocleavage;    Protein-DNA contact;   

DOI  :  10.1016/0014-5793(88)81245-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (λ ∼ 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the λ-repressor/O_R1 operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.

【 授权许可】

Unknown