Oncogenic ras protein induces meiotic maturation of amphibian oocytes in the presence of protein synthesis inhibitors

FEBS Letters

Santos, Eugenio²  Hinrichs, M.Victoria³  Allende, Catherine C.¹  Allende, Jorge E.² 

[1]Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, Chile

[2]Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA

[3]Departamento de Bioquímica, Facultad de Medicina, Universidad de Chile, Santiago, Chile

关键词: Maturation;    Protein-synthesis inhibitor;    Oocyte;    ras protein;   

DOI  :  10.1016/0014-5793(88)80130-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Microinjection of the activated ras oncogenic protein can induce the meiotic maturation of Xenopus laevis oocytes, a process that can also be triggered by progesterone or high concentrations of insulin. Cycloheximide and puromycin, well-known inhibitors of protein synthesis, block the maturation process induced by progesterone and insulin but do not affect the maturation caused by H-ras ^{lys 12} protein microinjection. Theophylline, an inhibitor of cAMP phosphodiesterase that also affects oocyte protein synthesis, does cause a partial inhibition of ras protein-induced maturation. These findings indicate that ras protein acts on the oocyte maturation process at a point that is downstream of the protein synthesis requirement, a characteristic shared with the maturation promoting factor, an activity that appears in oocytes and mitotic cells at the onset of cell division.

【 授权许可】

Unknown