Nucleotide sequence analysis of cDNAs encoding a bovine galanin precursor protein in the adrenal medulla and chemical isolation of bovine gut galanin

FEBS Letters

Carlquist, Mats²  Rökaeus, Åke¹ 

[1] Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD, USA;Depts of Biochemistry I and Biochemistry II, Karolinska Institutet, Stockholm, Sweden

关键词: Chromaffin cell;    Galanin message-associated peptide;    Hybridization histochemistry;    Peptide isolation;    RNA hybridization;   

DOI  :  10.1016/0014-5793(88)80125-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A description is given of the primary structure of a bovine adrenal medullary precursor protein (123 amino acids), containing a nonrepetitive galanin sequence, different in four amino acid positions from pig galanin, structural information deduced from the nucleotide sequence analysis of two cDNA clones. Pairs of lysine and arginine residues separate the galanin sequence N-terminally from the initiating methionine and a 29 amino acid leader peptide and C-terminally from a 59 amino acid peptide. The deduced amino acid composition was confirmed by the isolation and determination of the amino acid composition for bovine intestinal galanin. Northern blot analysis revealed the presence of an apparent single galanin mRNA species, of approx. 850–900 bases in size, which was present in a population of chromaffin cells scattered throughout the bovine adrenal medulla.

【 授权许可】

Unknown