Different effects of two thromboxane A2/prostaglandin H2 receptor ligands, U46619 and S‐145, on rabbit platelets

FEBS Letters

Nakano, Tohru¹  Hanasaki, Kohji¹  Arita, Hitoshi¹ 

[1] Shionogi Research Laboratories, Shionogi & Co., Ltd, Fukushima-ku, Osaka 553, Japan

关键词: Thromboxane A2;    Ca2+;    Shape change;    Phospholipase C;    U46619;    Inositol phosphate;    TXA2;    thromboxane A2;    PGH2;    prostaglandin H2;    IP3;    inositol 1;    4;    5-trisphosphate;   

DOI  :  10.1016/0014-5793(88)80105-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of rabbit platelets with U46619 induced platelet shape change, aggregation and secretion of ATP. However, S-145, which specifically binds to the thromboxane A₂/prostaglandin H₂ receptor like U46619, induced only shape change. Both compounds rapidly elevated cytoplasmic Ca²⁺ concentration although only U46619 evoked the formation of inositol phosphates. Chelating external Ca²⁺ with EGTA did not affect the S-145-induced platelet shape change while intracellular Ca²⁺ movement was severely reduced. These results suggest an essential role of phospholipase C in the induction of platelet aggregation and secretion and that some factor other than Ca²⁺ and phospholipase C participates in platelet shape change.

【 授权许可】

Unknown