Degradation of epidermal growth factor receptors by cathepsin L‐like protease: Inhibition of the degradation by c‐Ha‐ras gene products

FEBS Letters

Katunuma, Nobuhiko²  Yokoyama, Shigeyuki¹  Noguchi, Shigeru⁴  Bando, Yoshiaki²  Kominami, Eiki²  Sakiyama, Shigeru³  Ha, Jong-Myung¹  Hiwasa, Takaki³ 

[1] Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan;Department of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan;Division of Biochemistry, Chiba Cancer Center Research Institute, 666-2, Nitona-cho, Chiba 280, Japan;Biology Division, National Cancer Center Research Institute, 5-1-1 Tsukoi, Chuo-ku, Tokyo 104 Japan

关键词: c-Ha-ras;    Cathepsin L;    EGF receptor;    p21;    a c-Ha-ras;    gene product;    EGF receptor;    epidermal growth factor receptor;    Z-Phe-Arg-NMec;    benzyloxycarbonyl-phenylalanyl-arginine 4-methyl-7-coumarylamide;    Z-Arg-Arg-NMec;    benzyloxycarbonyl-arginyl-arginine 4-methyl-7-coumarylamide;    DTT;    dithiothreitol;    PMSF;    phenylmethylsulfonyl fluoride;    EGTA;    [ethylenebis(oxyethylenenitrilo)]-tetraacetic acid;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    E-64;    N-[N-(L-3-trans-car☐yoxiran-2-carbonyl)-L-leucyl]agmatine;    TLCK;    p-tosyl-L-lysine chloromethylketone;    TPCK;    p-tosyl-L-phenylalanine chloromethylketone;   

DOI  :  10.1016/0014-5793(88)80462-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extract of N1H3T3 mouse fibroblasts contains a protease which can cleave epidermal growth factor receptor (EGF receptor). This protease was tentatively named cathepsin X and purified to near homogeneity. The characteristics of cathepsin X were similar to those of cathepsin L and the proteolytic activity of cathepsin X was inhibited by c-Ha-ras gene products.

【 授权许可】

Unknown