Activation of c‐Ki‐ras coexists with c‐myc amplification in cells from a nude mouse tumor induced by the human breast carcinoma cell line SW 613‐S

FEBS Letters

Lavialle, Christian²  Champ, Brigitte³  Carloni, Guido²  Cassingena, Roland²  Vilarem, Marie-Jose´¹ 

[1] Laboratoire de Ge´ne´tique Cellulaire et Mole´culaire des Leuce´mies Aigu¨es, U301, INSERM, Hoˆpital Saint-Louis, Paris, France;Laboratoire de Ge´ne´tique Cellulaire, ER 278, CNRS, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France;Institut de Pathologie Mole´culaire, UA 1147, CNRS, Paris France

关键词: Breast carcinoma;    Gene activation;    Gene amplification;    ras gene;    myc gene;   

DOI  :  10.1016/0014-5793(88)80440-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In vitro transfection experiments have shown that cooperation between two different oncogenes can confer a fully malignant phenotype to primary rodent cells. We have previously reported that SW 613-Tul cells, derived from a tumor induced in a nude mouse by the human breast carcinoma cell line SW 613-S, showed a 30-fold amplification of the c- myc gene. In the present work, we show that these cells also harbor an activated c-Ki-ras gene capable of inducing the formation of foci upon transfection of NIH 3T3 cells with SW 613-Tul genomic DNA. Our results suggest that both the c-myc and c-Ki-ras oncogenes, activated by two different mechanisms, may cooperate in the full expression of the tumorigenic phenotype of SW 613-Tul cells.

【 授权许可】

Unknown