The location of CuA in mammalian cytochrome c oxidase

FEBS Letters

West, Ian C.¹  Rich, Peter R.¹  Mitchell, Peter¹ 

[1] Glynn Research Institute, Bodmin PL30 4 AU, England

关键词: Cytochrome c oxidase;    Energisation;    ATP effect;    Midpoint potential;    Proton pumping;   

DOI  :  10.1016/0014-5793(88)81349-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Imposition of a protonmotive force across the inner membrane of coupled cyanide-inhibited, beef heart mitochondria by addition of ATP causes reduction of cytochrome c and Cu_A with concomitant oxidation of haem a _A. The data are consistent with previous demonstrations of an intramembrane location of haem a _A but further indicate that Cu_A is very close to the cytosolic surface of the membrane. The implications of this finding for electron transfer route and the site of the proton pumping chemistry are discussed.

【 授权许可】

Unknown