Linolenic acid‐induced release of Mn, polypeptides and inactivation of oxygen evolution in photosystem II particles

FEBS Letters

Garstka, Maciej¹  Kaniuga, Zbigniew¹ 

[1] Institute of Biochemistry, University of Warsaw, Al. Zwirki i Wigury 93, PL 02-089 Warszawa, Poland

关键词: O2 evolution;    Mn;    Polypeptide;    Linolenic acid;    Photosystem II;    Chl;    chlorophyll;    C18:3;    linolenic acid;    Mes;    4-morpholineethanesulphonic acid;    PS II;    photosystem II;   

DOI  :  10.1016/0014-5793(88)80772-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The release of both Mn and polypeptides as well as the inactivation of O₂ evolution in C_18:3-treated PS II particles was studied. An increase of incubation temperature above 20°C greatly accelerated depletion of both Mn and peptides and inactivation of O₂ evolution. At 25°C about 30% of Mn was released whereas following incubation at 30°C release of 50% of Mn, complete depletion of both 10 and 18 kDa peptides as well as complete inactivation of O₂ evolution were observed. Simultaneously, the release of 24 and both 33 and 43 kDa peptides was about 40 and 30%, respectively. At 25°C, C_18:3 released only one Mn/reaction centre from PS II particles depleted of 18 and 24 kDa peptides, while two Mn/reaction centre were released from particles devoid of 18, 24 and 33 kDa peptides. These observations suggest that the 33 kDa peptide stabilizes preferentially two of the four Mn atoms of the oxygen-evolving complex. The effect of C_18:3 on Mn release from PS II particles is thus similar to that of heat and urea treatments.

【 授权许可】

Unknown