Derivation of the sequence of the signal peptide in human C4b‐binding protein and interspecies cross‐hybridisation of the C4bp cDNA sequence

FEBS Letters

Lintin, S.J.¹  Reid, K.B.M.¹  Lewin, A.R.² 

[1] MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU England;Imperial Cancer Research Fund, PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, England

关键词: Signal peptide;    C4b-binding protein;    Cross-hybridization;    Complement protein;    Evolution;   

DOI  :  10.1016/0014-5793(88)80763-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 5′ cDNA clone coding for human C4b-binding protein (C4bp) was isolated, characterised and sequenced to complete the cDNA sequence coding for residues 1–32 thus confirming the protein sequence data of Chung et al. [(1985) Biochem. J. 230, 133–141]. The sequence extended to allow derivation of the putative leader peptide sequence which was 32 residues in length and showed a high of hydrophobicity typical of other documented leader sequences. Cross hybridisation was detected between the human C4bp cDNA probes and genomic DNA isolated from various species on Southern blots suggesting that genomic sequence homologous to that coding for C4bp has been conserved during evolution.

【 授权许可】

Unknown