Structural requirements of choline derivatives for ‘conversion’ of pneumococcal amidase A new single‐step procedure for purification of this autolysin

FEBS Letters

Sanz, Jesus Miguel¹  Garcia, Jose Luis¹  Lopez, Rubens¹ 

[1] Unidad de Genetica Bacteriana, Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Velazquez 144, E-28006 Madrid, Spain

关键词: Choline;    DEAE-cellulose;    Amidase;    Autolysin;    (Pneumococcus);   

DOI  :  10.1016/0014-5793(88)80759-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tertiary amines appear to be the minimal structure needed to convert in vitro the inactive form (E-form) of pneumococcal amidase to the catalytic active form (C-form). Diethylethanolamine was one of the compounds that converted the E-form, a finding that has been used successfully to develop an affinity chromatography system in DEAE-cellulose for the rapid and efficient purification of lytic enzymes of pneumococcus and its bacteriophages.

【 授权许可】

Unknown