Bacillus thuringiensis ssp. galleriae simultaneously produces two δ‐endotoxins differing strongly in primary structure and entomocidal activity

FEBS Letters

Chestukhina, G.G.¹  Stepanov, V.M.¹  Kostina, L.I.¹  Khodova, O.M.¹  Zalunin, I.A.¹ 

[1] Institute of Genetics and Selection of Industrial Microorganisms, 113545 Moscow, USSR

关键词: Entomocidal crystal;    δ-Endotoxin;    (B. thuringiensis;    Diptera;    Lepidoptera;    Coleoptera);   

DOI  :  10.1016/0014-5793(88)80426-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Strain 11–67 of B. thuringiensis ssp. galleriae produces two entomocidal proteins of molecular mass 130 kDa. Limited proteolysis of these proteins — protoxins — yields the ‘true’ toxins of molecular mass 65 kDa which are drastically different with respect to their charges at pH 8.6, immunological properties and toxicity (host range). One of the proteins — the ‘negative’ component — is toxic for Lymantria dispar larvae and shows 65% homology when compared with B. thuringiensis ssp. kurstaki δ-endotoxins. The other — the ‘positive’ component — is toxic for Galleria mellonella larvae. Its N-terminal sequence of 11 amino acid residues is homologous with the B. thuringiensis ssp. israelensis and san diego endotoxins known to be toxic for Coleoptera and Diptera but not for Lepidoptera. Hence, B. thuringiensis subspecies may produce simultaneously δ-endotoxins differing substantially in structural features and host range.

【 授权许可】

Unknown