Heat‐stable microtubule protein MAP‐1 binds to microtubules and induces microtubule assembly

FEBS Letters

Maccioni, Ricardo B.¹  Rivas, Coralia I.¹  Vera, Juan C.¹ 

[1] Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, B-121, Denver, CO 80262, USA

关键词: Microtubule-associated protein;    Heat stability;    Affinity purification;    Microtubule assembly;   

DOI  :  10.1016/0014-5793(88)80408-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The microtubule-associated proteins, MAP-1, MAP-2 and tau, have been purified from brain tissue via a new approach using a heating step directly on the homogenate, followed by selective adsorption on calmodulin-Sepharose affinity columns and gel-filtration chromatography. Our results indicate that these MAPs share common biochemical properties, including heat stability, calmodulin binding and promotion of tubulin assembly into microtubules.

【 授权许可】

Unknown