A mutant LexA repressor harboring a cleavage motif cysteine‐glycine remains inducible

FEBS Letters

Schnarr, Manfred¹  Granger-Schnarr, Michèle¹  Oertel, Pascale¹ 

[1] Institut de Biologie Moléculaire et Cellulaire, CNRS and Université Louis Pasteur, 15, rue Descartes, 67084 Strasbourg Cédex, France

关键词: LexA repressor;    UmuD protein;    SOS induction;    Genotoxic agent;    Site-directed mutagenesis;   

DOI  :  10.1016/0014-5793(88)80866-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using site-directed mutagenesis of the lexA gene we have changed the amino acid Ala-84 of the LexA repressor for a cysteine. The reason for this change was the striking homology between LexA and UmuD and the comparable size of the two amino acid side chains. Using an in vivo repression/induction assay it is shown that the LexA-Cys-84 mutant remains inducible by mitomycin C and UV irradiation essentially in the same way as the wild-type repressor.

【 授权许可】

Unknown