Possible adsorption sites of cellulases on crystalline cellulose

FEBS Letters

Buleon, Alain¹  Henrissat, Bernard¹  Vigny, Béatrice¹  Perez, Serge¹ 

[1]Laboratoire de Biochimie des Protéines and Laboratoire de Physicochimie des Macromolécules, Institut National de la Recherche Agronomique, rue de la Géraudière, F-44072 Nantes, France

关键词: Cellulase;    Cellulose crystal;    Adsorption site;    Molecular graphics;   

DOI  :  10.1016/0014-5793(88)80726-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possible adsorption sites of cellulases on crystalline cellulose were investigated by molecular graphic representation of a crystal of cellulose and estimation of the accessibility of the various glycosidic bonds to enzymatic attack. The results show that only certain glycosidic bonds of certain surface cellulose chains are susceptible to enzymatic hydrolysis. These preferential sites correlate well with previous electron microscopy observations of the adsorption sites of 1,4-β-D-glucan cellobiohydrolase I (CBHI) from Trichoderma reesei on Valonia cellulose.

【 授权许可】

Unknown