Linear gramicidin activates neutrophil functions and the activation is blocked by chemotactic peptide receptor antagonist

FEBS Letters

Jacob, Jose¹ 

[1] Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India

关键词: Neutrophil;    Chemotactic peptide;    Gramicidin;    Chemotactic peptide receptor;    Superoxide;    Lysosomal enzyme;    FMLP;    formyl-methionyl-leucyl-phenylalanine methyl ester;    BMLP;    t-butyloxycarbonyl-methionyl-leucyl-phenylalanine;   

DOI  :  10.1016/0014-5793(88)80718-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activation of functional responses in rabbit peritoneal neutrophils by gramicidin and the chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine methyl ester, was studied. Gramicidin activated superoxide generation, lysosomal enzyme release and a decrease in fluorescence of chlortetracycline-loaded cells, as for the chemotactic peptide. The maximum intensities of the responses by gramicidin were lower than that by chemotactic peptide. Responses by both these peptides could be inhibited by t-butyloxycarbonyl-methionyl-leucyl-phenylalanine, a chemotactic peptide receptor antagonist. Gramicidin gave responses at low doses comparable to that of the chemotactic peptide.

【 授权许可】

Unknown