Gene expression of fibroblast matrix proteins is altered by indomethacin

FEBS Letters

Daireaux, M.²  Mauviel, A.²  Pujol, J.-P.²  Loyau, G.²  Kähäri, V.-M.³  Hartmann, D.J.¹  Heino, J.³ 

[1] Centre de Radioanalyse, Institut Pasteur, Lyon, France;Laboratoire de Biochimie du Tissu Conjonctif, Université de Caen, CHU, Côte de Nacre, F-14040 Caen Cedex, France;Department of Medical Biochemistry, University of Turku, Turku, Finland

关键词: Indomethacin;    Collagen;    Fibronectin;    Dermatan sulfate proteoglycan core protein;    Prostaglandin E2;    Extracellular matrix;   

DOI  :  10.1016/0014-5793(88)80715-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of indomethacin in the regulation of extracellular matrix synthesis was studied in dermal fibroblast cultures. Indomethacin (10 μM) blocked totally the prostaglandin secretion and markedly increased the synthesis of collagen. In parallel, measurement of fibronectin, type I and type III procollagen mRNA levels showed a substantial increase under the action of indomethacin. On the other hand, indomethacin did not modify the mRNA levels of dermatan sulfate proteoglycan core protein. Measurement of collagen production estimated as the amount of collagenase digestible protein and by specific radioimmunoassay indicated a good correlation with the corresponding mRNA levels. These results suggest that indomethacin can regulate the extracellular matrix deposition at a transcriptional level.

【 授权许可】

Unknown