Quantitation and identification of carbon‐13 isotopomers by homonuclear polarization transfer NMR experiments

FEBS Letters

Boudot, Denis¹  Brondeau, Jean¹  Canet, Daniel¹  Martin, Francis² 

[1] Laboratoire de Méthodologie RMN (UA CNRS no 406 - LESOC), Université de Nancy I, BP no 239, 54506 Vandoeuvre-les-Nancy Cedex, France;Laboratoire de Microbiologie Forestière (CRF), Institut National de la Recherche Agronomique, Champenoux 54280 Seichamps, France

关键词: 13C isotopomer;    NMR;    Polarization transfer;    Metabolic pathway;    Ectomyocorhizal fungus;   

DOI  :  10.1016/0014-5793(88)80692-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The simplest pulse sequence (π/2)_x—τ— (π/2)_y (Acquisition) for nuclear spin polarization transfer is used in a selective fashion: transfer only flows from a nucleus A to any nucleus X J-coupled to A, provided that the carrier frequency coincides with the A resonance and that τ is slightly varied around a properly chosen value, relevant free induction decays being co-added. According to the value selected for τ (1/2J or 1/4J, J: one bond carbon-carbon coupling constant), it proves possible to sort out and compare subspectra pertaining to isotopomers involving one, two, three, and four consecutive ¹³C. The method has been applied to an extract of mycelium grown on a medium containing ¹³C-labeled acetate, with the aim of delineating metabolic pathways from proportions of the detected isotopomers.

【 授权许可】

Unknown