Muscarinic receptor‐mediated intracellular Ca2+ mobilization in embryonic chick heart cells

FEBS Letters

Oettling, Günter¹  Drews, Ulrich¹  Schmidt, Heinrich¹ 

[1] Anatomisches Institut der Universität Tübingen, Österbergstraße 3, D-7400 Tübingen, FRG

关键词: Ca2+ mobilization;    Muscarinic receptor;    Chlortetracycline;    (Chick heart);    IP3;    myo-inositol 1;    4;    5-trisphosphate;    CTC;    chlortetracycline;   

DOI  :  10.1016/0014-5793(88)80636-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of muscarinic receptors of heart cells elevates the intracellular Ca²⁺ concentration. The increase is considered to be due to influx of extracellular Ca²⁺. We show that intracellular Ca²⁺ mobilization is involved. Cell suspensions prepared from hearts of 6-day-old chick embryos were loaded with the fluorescent Ca²⁺ chelator chlortetracycline. Muscarinic stimulation induces a dose-dependent fluorescence decrease (ED₅₀=2.6 × 10⁻⁶ M) indicating intracellular Ca²⁺ mobilization.

【 授权许可】

Unknown