Characterization of an isolated chloroplast membrane Fe◀S protein and its identification as the photosystem I Fe◀SA/Fe◀SB binding protein

FEBS Letters

Malkin, Richard¹  Wynn, R.Max¹ 

[1] Division of Molecular Plant Biology, University of California, Berkeley, CA 94720, USA

关键词: Iron-sulfur center;    Photosystem I;    Ferredoxin;    P700;   

DOI  :  10.1016/0014-5793(88)81143-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An iron-sulfur protein of approx. 9 kDa has been isolated from spinach chloroplast membranes. Based on iron and sulfide content (∼900 nmol Fe and S²⁻ per mg protein), optical absorbance and low-temperature EPR spectra, it appears that this protein contains 8Fe and 8S²⁻ in two [4Fe◀4S] clusters. The protein cross-reacts with an antibody raised against a 9 kDa PS I subunit previously identified as the psaC (frxA) gene product. This identity was confirmed by the N-terminal amino acid sequence of the isolated Fe◀S protein. It is concluded that the isolated Fe◀S protein binds PS I centers Fe◀S_A and Fe◀S_B and that each of these centers contains 4Fe and 4S²⁻.

【 授权许可】

Unknown