2‐Mercaptoethylamine, a competitive inhibitor of spermidine synthase in mammalian cells

FEBS Letters

Kawase, Masahiro¹  Nakashima, Kunio²  Hibasami, Hiroshige²  Sakurai, Masaki²  Tsukada, Tetsuya²  Maekawa, Satoru² 

[1] Yamanaka Hospital of Gastroenterology, Yokkaichi, Mie 510, Japan;Department of Biochemistry, Mie University School of Medicine, Tsu, Mie 514 Japan

关键词: 2-Mercaptoethylamine;    Spermidine synthase inhibitor;    Polyamine depletion;    (Human leukemia cell);    MEA;    2-mercaptoethylamine;    MTA;    5′-methylthioadenosine;    decarboxylated AdoMet;    decarboxylated S-adenosylmethionine;   

DOI  :  10.1016/0014-5793(88)81133-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spermidine synthase from rat ventral prostate was inhibited by 2-mercaptoethylamine (MEA). Inhibition of spermidine synthase by MEA was competitive with respect to one of the substrates putrescine, but not competitive with respect to the other substrate decarboxylated S-adenosylmethionine. MEA markedly depressed spermidine and spermine contents in human erythroid leukemia K562 cells, suggesting that these changes resulted from the inhibitory effect of MEA on spermidine synthase in situ.

【 授权许可】

Unknown