Adsorption of cytochrome c to phospholipid monolayers studied by reflection spectroscopy

FEBS Letters

Kozarac, Z.¹  Möbius, D.²  Dhathathreyan, A.² 

[1] Center for Marine Research, Rudjer Boskovic Institute, Zagreb, Croatia, Yugoslavia;Max-Planck-Institut für Biophysikalische Chemie, Postfach 2841, D-3400 Göttingen, FRG

关键词: Cytochrome c;    Phospholipid monolayer;    Reflection spectroscopy;   

DOI  :  10.1016/0014-5793(88)81159-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The uptake of cytochrome c by charged and neutral lipid monolayers was studied by using reflection spectroscopy. The method was shown to be a very sensitive and useful technique in studies of lipid-protein interactions. It was found that cytochrome c is preferentially bound to monolayers of negatively charged monolayers in the solid phase. Polarized light under oblique incidence was used to determine the average orientation of chromophores in cytochrome c bound to lipid monolayer. The transition moments of chromophore are oriented parallel to the monolayer plane.

【 授权许可】

Unknown