A resonance Raman study of the binding of ethanol and methanol to ferrihemoglobin

FEBS Letters

Muhoberac, B.B.³  Ondrias, M.R.¹  Shelnutt, J.A.² 

[1] Department of Chemistry, University of New Mexico, Albuquerque, NM 87131, USA;Process Research Division, Sandia National Laboratories, Albuquerque, NM 87185 USA;Department of Chemistry, Purdue University School of Science, Indiana University-Purdue University at Indianapolis, Indianapolis, IN 46223, USA

关键词: Ferrihemoglobin;    Ethanol;    Methanol;    Resonance Raman spectroscopy;    Ligand binding;    (Methemoglobin);   

DOI  :  10.1016/0014-5793(88)80022-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interactions of ethanol and methanol with ferrihemoglobin were examined using resonance Raman spectroscopy. After binding either alcohol, the low-frequency resonance Raman spectra of human ferrihemoglobin are almost identical to the unperturbed spectrum except for shifts in the 309 cm⁻¹ band to higher frequency by as much as 8 cm⁻¹. The ethanol-induced shift is greater than that with methanol even though complex formation was less for ethanol than methanol. The spectral changes imply a site-specific, similar binding of these alcohols to ferrihemoglobin which may involve steric interactions. Possible assignments of the 309 cm⁻¹ band to structural features as well as potential mechanisms of the alcohol-induced spectral changes are discussed.

【 授权许可】

Unknown