Isolation of the intact photosystem I reaction center core containing P700 and iron‐sulfur center FX

FEBS Letters

Brand, Jerry J.¹  Mehari, Tetemke²  Jones, Karen L.²  Parrett, Kevin G.²  Golbeck, John H.² 

[1] Department of Botany, University of Texas, Austin, TX 78713, USA;Department of Chemistry, Portland State University, Portland, OR 97207, USA

关键词: Photosynthesis;    Reaction center;    Photosystem I;    P700;    Iron-sulfur center FX;    Iron-sulfur center FA;    Iron-sulfur center FB;    Chl;    chlorophyll;    LDS;    lithium dodecyl sulfate;    DCPIP;    2;    6-dichlorophenolindophenol;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(88)80013-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The photosystem I reaction center core containing P700 and iron-sulfur center F_X has been isolated from a Synechococcus photosystem I particle with 6.8 M urea at pH 10.0 followed by sucrose density ultracentrifugation. The reaction center core has retained > 90% of F_X and 100% of P700 (determined by optical spectroscopy) but is totally devoid of iron-sulfur centers F_A and F_B (determined by optical and ESR spectroscopy). SDS-PAGE indicates the retention of the 57 kDa reaction center polypeptide(s) but the total absence of the 16.4 and 8.1 kDa polypeptides. The loss of F_A and F_B is further reflected in the decline of acid-labile sulfide from 11.8 ± 0.4 S²⁻ /P700 in the control particle to 4.6 ± 0.3 S²⁻ /P700 in the reaction center core. This preparation represents the first isolation of an intact reaction center core incorporating the components P700 and F_X but totally lacking F_A and F_B.

【 授权许可】

Unknown