Iodinated melatonin mimics melatonin action and reveals discrete binding sites in fetal brain

FEBS Letters

Reppert, Steven M.²  Weaver, David R.²  Namboodiri, M.A.Aryan¹ 

[1] Department of Biology, Georgetown University, Washington, DC 20057, USA;Laboratory of Developmental Chronobiology, Children's Service, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114 USA

关键词: Melatonin;    Circadian rhythm;    Photoperiodism;    Reproduction;    (Hypothalamus;    Suprachiasmatic nucleus);   

DOI  :  10.1016/0014-5793(88)80599-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Iodinated melatonin was used to study melatonin sites of action in brain. Iodomelatonin mimicked the effects of melatonin on reproductive development in Djungarian hamster fetuses. ¹²⁵I-melatonin injected into the dam was recovered from fetal brain. In vitro autoradiographic studies revealed a remarkably discrete distribution of competitive ¹²⁵I-melatonin-binding sites in the fetal brain, with binding in median eminence/arcuate nucleus area > suprachiasmatic nucleus > pineal gland ⪢ anterior pituitary gland ⪢ preoptic area. ¹²⁵I-melatonin promises to be a useful tool for understanding the sites and mechanism of action of melatonin.

【 授权许可】

Unknown