Nicotinic acetylcholine receptor: A structural model for α‐subunit peptide 188–201, the putative binding site for cholinergic agents

FEBS Letters

Clementi, F.²  Bolognesi, M.⁴  Frigerio, F.⁴  Racchetti, G.³  Longhi, R.¹  Gotti, C.² 

[1] CNR Institute of Hormone Chemistry, Recordati Spa, Milan, Italy;Department of Medical Pharmacology, University of Milan, Recordati Spa, Milan, Italy;Section of Biochemistry and Immunology, Recordati Spa, Milan, Italy;Department of Genetics, University of Pavia, Recordati Spa, Milan, Italy

关键词: Nicotinic acetylcholine receptor;    α-Bungarotoxin;    Monoclonal antibody;    Computer graphics;    Molecular modeling;    α-Toxin binding site;   

DOI  :  10.1016/0014-5793(88)80598-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A peptide corresponding to amino acid sequence 188–201 of the α-subunit of Torpedo AChR binds α-Bgtx. The S-S bridge between Cys 192 and 193 is essential for the binding as Tyr in position 189. The same sequence 188–201 corresponding to human AChR, which instead of Tyr has a Thr in position 189, binds α-Bgtx with a much lower efficiency. Monoclonal antibodies raised against Torpedo peptide 188–201 recognize Torpedo AChR and antibodies against Torpedo AChR recognize peptide 188–201 indicating that the synthetic peptide and the corresponding sequence in the native molecule share some immunological epitopes. With computer graphics and energy refinement a molecular model of this peptide has been elaborated.

【 授权许可】

Unknown