Transforming growth factor β regulates the levels of different fibronectin isoforms in normal human cultured fibroblasts

FEBS Letters

Zardi, Luciano¹  Balza, Enrica¹  Borsi, Laura¹  Allemanni, Giorgio¹ 

[1] Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, Viale Benedetto XV, 10, 16132 Genova, Italy

关键词: Transforming growth factor β;    Fibronectin isoform;    RNA splicing;   

DOI  :  10.1016/0014-5793(88)80580-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fibronectin (FN) polymorphism is caused by alternative splicing patterns in at least three regions of the primary transcript of a single gene. Using a monoclonal antibody (Mab) specific for an FN segment (ED-A), that can be included or omitted from the molecule depending on the pattern of splicing, we have examined whether transforming growth factor β (TGF-β) and dexamethasone, which are both known to increase the level of total FN, regulate the levels of different FN isoforms. We found that, while dexamethasone does not significantly change the ratio between the total FN and the ED-A containing FN, TGF-β preferentially increases the expression of the FN isoform containing the ED-A sequence.

【 授权许可】

Unknown