Catalytic subunit of cAMP‐dependent protein kinase is essential for cAMP‐mediated mammalian gene expression

FEBS Letters

Jastorff, B.¹  Walter, U.²  Büchler, W.²  Lohmann, S.M.² 

[1] Bio-organische Chemie, Universität Bremen, 2800 Bremen, FRG;Labor für Klinische Biochemie, Medizinische Universitätsklinik, Josef-Schneider-Str. 2, 8700 Würzburg, FRG

关键词: Protein kinase subunit inhibitor;    cyclic AMP-stimulated transcription;    Tyrosine aminotransferase;    Phosphoenolpyruvate carboxykinase;    (Hepatocyte);   

DOI  :  10.1016/0014-5793(88)80577-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cyclic AMP-stimulated mRNA levels in cultured rat hepatocytes were inhibited by three different inhibitors of cAMP-dependent protein kinase activity: (i) Rp-cAMPS, a cAMP analog with a sulfur substitution at the equatorial oxygen of the cyclic monophosphate; (ii) H8, an isoquinoline sulfonamide derivative; and (iii) PKI, a 20-amino acid synthetic peptide of the Walsh protein kinase inhibitor. These inhibitors specifically blocked the cAMP-stimulated increase in mRNA for tyrosine aminotransferase and phosphoenolpyruvate carboxykinase; they had no effect on the level of albumin mRNA which is not cAMP regulated. These results provide functional evidence that kinase activity involving protein phosphorylation is required in cAMP-mediated gene expression in mammalian cells.

【 授权许可】

Unknown