Cloning, sequencing and expression of a full‐length rabbit fast skeletal troponin‐C cDNA

FEBS Letters

Gergely, John²  Taljanidisz, Janos¹  Tao, Terence²  Chen, Qian²  Sarkar, Satyapriya³ 

[1] Department of Metabolic Regulation, Boston Biomedical Research Institute, Tufts University School of Medicine, Boston, Massachusetts, USA;Department of Muscle Research, Tufts University School of Medicine, Boston, Massachusetts, USA;Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts, USA

关键词: Troponin-C;    cDNA;    Nucleotide sequence;    cDNA expression;    (E. coli);    TnC;    rabbit fast skeletal troponin-C;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(88)80576-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length cDNA coding for troponin-C isolated from an adult rabbit fast skeletal muscle library has been sequenced and expressed in an E. coli host. The amino acid sequence derived from the coding region agrees with the published protein sequence except that the first two residues are reversed. The expressed protein was found to be identical to purified rabbit skeletal troponin-C based on electrophoretic mobilities in polyacrylamide gels containing either SDS or urea, and on immunoblotting. These results establish that our troponin-C cDNA clone is suitable for site-directed mutagenesis studies on the structure and function of troponin-C

【 授权许可】

Unknown