An initiator protein for plasmid R6K DNA replication Mutations affecting the copy‐number control

FEBS Letters

Wada, Yukie¹  Inuzuka, Manabu¹ 

[1] Department of Biochemistry, Fukui Medical School, Matsuoka, Fukui 910-11, Japan

关键词: Plasmid R6K;    DNA replication;    Initiator protein;    Copy mutant;    Temperature-sensitive mutation;    Nucleotide sequence;   

DOI  :  10.1016/0014-5793(88)80573-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two kinds of mutations affecting the copy-number control of plasmid R6K were isolated and identified in an initiator π protein by DNA sequencing. Firstly, a temperature-sensitive replication mutation, ts22, with decreased copy number results in a substitution of threonine to isoleucine at position 138 of the 305-amino-acid π protein. Secondly, a high-copy-number (cop21) mutant was isolated from this ts mutant and was identified by an alteration of alanine to serine at position 162. This cop21 mutation suppressed the Ts character and was recessive to the wild-type allele in the copy control.

【 授权许可】

Unknown