GTPγS activation of proto‐oncogene expression in transiently permeabilised Swiss 3T3 fibroblasts

FEBS Letters

Metcalfe, James C.¹  Pennington, Stephen R.¹  Hesketh, T.Robin¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: c-fos;    c-myc;    Guanine nucleotide;    Phosphoinositide hydrolysis;    cyclic AMP;    EGF;    epidermal growth factor;    GTPγS;    guanosine 5′-O-(3-thiotriphosphate);    IBMX;    3-isobutyl 1-methylxanthine;    Ins(1;    4;    5)P3;    D-myo-inositol 1;    4;    5-trisphosphate;    PBS;    phosphate-buffered saline;    PtdIns(4;    5)P2;    1-(3-sn-phosphatidyl)-D-myo-inositol 4;    5-bisphosphate;   

DOI  :  10.1016/0014-5793(88)80899-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A technique of transient permeabilisation has been used to show that the introduction of guanosine 5′-O-(3-thiotriphosphate) (GTPγS), a non-hydrolysable analogue of GTP, into intact Swiss 3T3 fibroblasts stimulates phosphoinositide hydrolysis, cyclic AMP accumulation and the activation of c-fos and c-myc proto-oncogenes. Of a number of nucleotide triphosphates introduced into the cells, only GTP and its non-hydrolysable analogues activated inositol phosphate release, suggesting that this response is mediated by guanine nucleotide regulatory (G) protein(s). The data demonstrate that transient permeabilisation provides a method of examining the involvement of G-proteins in nuclear activation.

【 授权许可】

Unknown