Mechanism of transport of L‐alanine by luminal‐membrane vesicles from pars recta of rabbit proximal tubule

FEBS Letters

Sheikh, M.Iqbal¹  Jessen, Henrik¹  Jørgensen, Karl Evald¹  Vorum, Henrik¹ 

[1] Institute of Medical Biochemistry, University of Aarhus, DK-8000 Aarhus C, Denmark

关键词: Transport of L-alanine;    Na+ dependence;    pH dependence;    Chemical modification;    Pars recta;    (Rabbit kidney);   

DOI  :  10.1016/0014-5793(88)81408-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The characteristics of renal transport of L-alanine by luminal-membrane vesicles from proximal straight tubules (pars recta) of rabbit kidney were investigated. The following picture emerges from transport studies. Two electrogenic and Na⁺ requiring systems confined to this region of the nephron exist for the transport of L-alanine. In addition to Na⁺, the transport of L-alanine was influenced by H⁺. However, H⁺ does not substitute for Na⁺, but instead potentiates the Na⁺ effect. Modification of histidyl residues of the intact luminal-membrane vesicles by diethylpyrocarbonate (DEP), completely abolished the transient renal accumulation of L-alanine. Substrate and Na⁺-protection experiments suggest that histidyl residues may be at or close to the active site of the L-alanine transporter in membrane vesicles from pars recta.

【 授权许可】

Unknown